Theory 15/Seconds to Minutes/Metabolic control
Control Needs a Fast Enzyme
The claim
Metabolic flux control is set by the ratio of enzyme response time to the timescale of flux change, so a slow allosteric response cannot regulate a fast flux.
01Mechanism
Regulation requires the enzyme to respond within the period over which substrate changes; beyond the ratio, the response lags and overshoots.
02Prediction
Control should degrade as the ratio rises.
03Refutation
Control tracking enzyme amount would remove response time.