Theory 15/Nanoscale/Nanopore sequencing
Signal Is Pore Against Base
The claim
Nanopore current blockade is set by the ratio of pore diameter to nucleotide size, so a tight pore reads more distinctly but blocks more often.
01Mechanism
The blockade depends on how much of the pore the nucleotide occupies; the ratio sets both signal and residence.
02Prediction
Signal-to-noise should peak at an intermediate ratio.
03Refutation
Signal increasing with pore size would remove occupancy.