Theory 02/Molecular/Enantioselectivity
Chirality Needs a Stiff Site
The claim
Enzyme enantioselectivity is set by the ratio of transition-state geometry to active-site flexibility, so a rigid site discriminates better than a flexible one of equal affinity.
01Mechanism
Discrimination requires the two enantiomers to differ in how they fit; flexibility averages that difference away.
02Prediction
Selectivity should rise as flexibility falls.
03Refutation
Selectivity tracking affinity would remove flexibility.